Enzymes Metabolism and Cellular Energetics Practice Questions | A-Level Biology

Enzymes Metabolism and Cellular Energetics

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In a purified enzyme system, an inhibitor causes the maximum reaction rate to decrease, while substrate affinity appears unchanged when measured over a wide substrate concentration range. Which inference is most consistent with this observation?

Select one option.

The inhibitor reduces the effective concentration of functional enzyme molecules without preventing substrate binding
The inhibitor binds only to the enzyme substrate complex and increases substrate affinity
The inhibitor competes with substrate at the active site and can be fully displaced by excess substrate
The inhibitor increases substrate binding while increasing the maximum rate
The inhibitor raises temperature sensitivity without affecting catalysis

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